Effects of modification of mineral trioxide aggregate with zeolite-clinoptilolite on physiochemical, antibacterial characteristics and marginal adaptation in apical obturation of single-rooted teeth

[S. S. Veličković]

89 listova

Uvod: Hronične periapikalne lezije su patološke lezije lokalizovane u predelu vrha korena zuba sa avitalnom pulpom. U slučajevima kada endodontska terapija ne pokaže rezultate potrebno je sprovesti i periapikalnu hiruršku terapiju. Cilj ove studije bio je da se proceni uticaj modifikacije mineral trioksid agregata zeolitomklinoptilolitom na fizičkohemijske i antibakterijske karakteristike, imunomodulatorna svojstva, bikompatibilnost i rubno zaptivanje u apeksnoj opturaciji jednokorenih zuba. Materijal i metode: Ovo istraživanje predstavlja eksperimentalnu studiju na uzorcima cementa i in vitro studiju na izvađenim jednokorenim zubima. Modifikacija MTA cementa pripremljena je dodavanjem (5% od ukupnog masenog udela) prirodnog zeolita-klinoptilolita i modifikovanog zeolita klinoptilolita ZrO₂ ili Ag i Zn u prah komponentu MTA. Rezultati: Modifikacija cementa ima uticaj na fizičkohemijske osobine (statički značajna razlika između grupa p<0,001, p=0,001), antibakterijske karakteristike (grupa 3 i grupa 4 su pokazali najefikasnije antimikrobno dejstvo protiv E. faecalis), imunomodulatorna svojstva (grupa 2 smanjuje koncentraciju IL-1β, TNF-α, IFN-γ, IL-17) i biokompatibilnost (nakon 72 sata, grupa 2 je pokazala statistički značajno poboljšanje vijabilnosti ćelija). Prosečne veličine mikropukotina pukotina nisu bile statistički različite p>0.05. Zaključak: Uzimajući u obzir njegov uticaj na fizičkohemijske karakteristike i rubno zaptivanje, kao i biokompatibilnost, antibakterijska i imunomodulatorna svojstva, dodavanje prirodnog zeolita u MTA predstavlja potencijal za razvoj novih materijala za retrogradnu opturaciju.

Introduction: Periapical lesions are pathological lesions localized in the region of the root apex of teeth with non-vital pulp. In cases where endodontic therapy does not yield satisfactory results, periapical surgical treatment is required. The aim of this study was to evaluate the effect of modification of mineral trioxide aggregate with zeolite–clinoptilolite on physicochemical and antibacterial characteristics, immunomodulatory effects, biocompatibility, and marginal adaptation in apical obturation of single-rooted teeth. Materials and Methods: This research represents an experimental study on cement samples and an in vitro study on extracted single-rooted teeth. Modification of MTA cement was prepared by adding 5% (of the total mass fraction) of natural zeolite–clinoptilolite and modified zeolite–clinoptilolite ZrO₂ or Ag and Zn to the powder component of MTA. Results: Effect of cement modification on physicochemical properties (statistically significant differences between groups, p < 0.001, p = 0.001), antibacterial characteristics (Groups 3 and 4 demonstrated the most effective antimicrobial activity against E. faecalis), immunomodulatory properties (Group 2 reduced the concentrations of IL-1β, TNF-α, IFN-γ, and IL-17), and biocompatibility (after 72 hours, Group 2 showed a statistically significant improvement in cell viability). The mean sizes of microleakage were not statistically different (p > 0.05). Conclusion: Considering its influence on physicochemical characteristics and marginal adaptation, as well as biocompatibility, antibacterial, and immunomodulatory properties, the addition of natural zeolite to MTA represents a potential for the development of novel rootend filling material.

materijal za retrogradnu opturaciju, mineral trioksid agregat, fizičkohemijske osobine, E. faecalis, biokompatibilnost, imunomodulacija, mikrocurenje, zeolit.

root-end filling material, mineral trioxide aggregate, physicochemical properties, E. faecalis, biocompatibility, immunomodulatory effects, microleakage, zeolite.

616.31(497.11)

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