Synthesis, characterization and antimicrobial activity of polinuclear silver(I) complexes with aromatic nitroden-donor ligands

[S. Đurić]

87 listova

U ovoj doktorskoj disertaciji opisana je sinteza, strukturna karakterizacija i biološka aktivnost osam kompleksa srebra(I) sa različitim aromatičnim azotdonorskim ligandima (1,10-fenantrolin, 5,6-epoksi-5,6-dihidro-1,10-fenantrolin, 1,5-naftiridin, 1,2-bis(4-piridil)etan i 1,2-bis(4-piridil)eten). U cilju ispitivanja uticaja kontra-anjona na strukturne karakteristike kompleksa srebra(I), u njihovoj sintezi su korišćene različite soli srebra(I) (AgNO3, AgCF3SO3 i AgCF3COO). Ispitivana je antimikrobna aktivnost sintetisanih kompleksa srebra(I) prema različitim bakterijama i gljivicama, kao i njihov citotoksični efekat na normalnoj ćelijskoj liniji fibroblasta pluća (MRC-5). U pojedinim slučajevima, određena je antitumorska aktivnost kompleksa na osnovu in vitro ispitivanja citotoksičnosti na različitim ćelijskim linijama tumora, kao i embriotoksičnost na in vivo modelu zebra ribica (Danio rerio). U cilju definisanja afiniteta sintetisanih kompleksa srebra(I) prema biološki značajnim molekulima, ispitivane su njihove interakcije sa nukleinskim kiselinama (DNA) i proteinima (albumin goveđeg seruma, BSA). U prvom delu doktorske disertacije prikazan je značaj kompleksa različitih jona metala u medicini sa posebnim osvrtom na komplekse srebra(I) koji pokazuju značajnu antimikrobnu i antitumorsku aktivnost. U drugom delu disertacije detaljno su opisani postupci za sintezu kompleksa i metode za njihovu strukturnu karakterizaciju i biološko ispitivanje. U delu disertacije koji se odnosi na diskusiju rezultata prikazani su rezultati spektroskopske i kristalografske karakterizacije sintetisanih kompleksa, rezultati dobijeni ispitivanjem njihove antimikrobne i antitumorske aktivnosti i toksičnosti, kao i ispitivanjem njihovih interakcija sa biološki značajnim molekulima.

This doctoral dissertation describes the synthesis, structural characterization and biological activity of eight silver(I) complexes with different aromatic nitrogen-donor ligands (1,10-phenanthroline, 5,6-epoxy-5,6-dihydro-1,10-phenanthroline, 1,5-naphthyridine, 1,2- bis(4-pyridyl)ethane and 1,2 -bis(4-pyridyl)ethene). In order to investigate the influence of the counter anions on the structural characteristics of the synthesized silver(I) complexes, different silver(I) salts (AgNO3, AgCF3SO3 and AgCF3COO) were used in their synthesis. The antimicrobial activity of the synthesized silver(I) complexes was studied against various bacterial and fungal strains, as well as their cytotoxic effect on the normal human lung fibroblast cell line (MRC-5). In particular cases, the antitumor activity of the complexes was determined based on the evaluation of their in vitro cytotoxicity on different human tumor cell lines, as well as embryotoxicity in vivo in the zebrafish model (Danio rerio). In order to determine the affinity of the synthesized silver(I) complexes towards biologically important molecules, their interactions with nucleic acids (DNA) and proteins (bovine serum albumin, BSA) were investigated. In the first part of this doctoral dissertation, the importance of metal complexes in medicine is presented, with special attention on the silver(I) complexes that have shown significant antimicrobial and antitumor activities. In the second part of the dissertation, the procedures for the synthesis of complexes and methods for their structural characterization and biological evaluation are described in detail. The results of the spectroscopic and crystallographic characterization of the synthesized complexes and the results obtained by testing their antimicrobial and antitumor activity and toxicity, as well as studying their interactions with biologically important molecules, are presented in the part of the dissertation related to the discussion of the results.

546.571-386(043.3)

Srebro, jednovalentno - Kompleksna jedinjenja

Tekst