Development of topical essential oil (Helichrysum italicum) and investigation of their potential in the treatment of wounds and inflammation

[M. M. Anđić]

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Cilj istraživanja bio je razvoj i karakterizacija formulacija sa etarskim uljem Helichrysum italicum i ispitivanje bezbednosti i efikasnosti lokalne primene datih formulacija. Ispitivanje bezbednosti sprovedeno prema OECD smernicama pokazalo je da su mast i gel na bazi etarskog ulja smilja bezbedni za dermalnu primenu. U skladu sa tim, dalje je sprovedeno ispitivanje lokalnog antiinflamacijskog potencijala formulacija na bazi smilja na modelu karagenanom indukovane inflamacije. Sposobnost antiinflamacijske aktivnosti formulacija smilja je procenjena praćenjem razvoja edema šape, ali i markera inflamacije i oksidacionog stresa. Rezultati pokazuju da topikalne formulacije na bazi etarskog ulja smilja ispoljavaju značajan inhibitorni efekat na povećanje edema šapa pacova. Dodatno, primećena je značajna modulacija koncentracije markera inflamacije i oksidacionog stresa u grupama tretiranim formulacijama na bazi smilja. Efikasnost formulacija u procesu zarastanja rana ispitivana je na modelima rana formiranih metodom ekscizije i incizije. Odgovor na tretman je procenjen makroskopskom, biohemijskom i histopatološkom analizom. Lokalna primena masti i gela na bazi ulja smilja pokazala je najveću kontrakciju rane sa najvećim sadržajem hidroksiprolina i u modelu incizije i modelu ekscizije. Bolja sposobnost reepitelizacije preparata sa smiljem ogleda se i u histopatološkim rezultatima koji ukazuju na povećanu gustinu kolagenih vlakana. Dobijeni rezultati posledica su i antiiflamacijske i antioksidacione aktivnosti koja je pokazana detektovanjem datih markera u homogenatu tkiva. Naši rezultati ukazuju da bi primena formulacija na bazi etarskog ulja smilja bile obećavajuće i inovativno sredstvo kako za lečenje lokalne inflamacije tako i za zarastanje rana.

The aim was the development and characterization of formulations based on Helichrysum italicum essential oil and testing the safety and effectiveness of their local application. A safety study conducted according to OECD guidelines showed that the formulations based on immortelle essential oil are safe for dermal application. Accordingly, the local antiinflammatory potential of immortelle-based formulations was investigated in the carrageenaninduced inflammation model. The ability of anti-inflammatory activity was evaluated by monitoring the development of paw edema, as well as markers of inflammation and oxidative stress. The results have shown that topical formulations based on immortelle essential oil exert a significant inhibitory effect on the increase in rat paw edema. Additionally, a significant modulation of the concentration of markers of inflammation and oxidative stress was observed in groups treated with immortelle oil-based formulations. The effectiveness of the formulations in the wound healing process was examined on wounds created by the excision and incision. Response to treatment was assessed by macroscopic, biochemical, and histopathological analysis. Topical application of ointment and gel based on immortelle oil showed the greatest contraction of the wound with the highest content of hydroxyproline in both models. The better re-epithelialization ability is also observed via histopathological results, indicating an increased density of collagen fibers. The obtained results are the consequence of both anti-inflammatory and antioxidant activity, demonstrated by detecting the markers in the tissue homogenate. Our results indicate that immortelle formulations could be a promising tool for both local inflammation and wound healing.

Helichrysum italicum; etarsko ulje; zarastanje rana; inflamacija; oksidacioni stres

Helichrysum italicum; esssential oil; wound healing; inflammation; oxidative stress.

615(043.3)

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