Тhe role of COPB2 expression and the process of apoptosis in the genesis of endometrium

[B. M. Andrić]

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U nastanku karcinoma endometrijuma važnu ulogu imaju geni zaduženi za sintezu proteina koji učestvuju u ćelijskim tarnsportnim i metaboličkim procesima. Proteini familije Bcl-2 ključni su unutarćelijski modulatori procesa apoptoze. U do sada rađenim studijama dokazan je značaj COPB2 u nastanku i razvoju adenokarcinoma. Cilj ove studije je ispitati uticaj poremećaja apoptoze i ekspresije gena za COPB2 na nastanak karcinoma endometrijuma. Uzimalo se tkivo endometrijuma od pacijentkinja eksperimentalne (40 ispitanica) i kontrolne grupe (20 ispitanica). Metodom reverzne transkripcije kvantitativnog PCR-a određivala se relativna ekspresija gena za COPB2 i protein Bcl-2 u endometrijalnim ćelijama. Ispitana je uloga poremećaja regulacije apoptoze preko Bcl-2, kao i ekspresije gena za COPB2 u nastanku karcinoma endometrijuma. Utvrđeno je da postoji statistički značajna razlika (R<0,005) u vrednostima ekspresije gena COPB2 i Bcl-2 između pacijentkinja sa i bez karcinoma endometrijuma. Za oba gena je prikazan značajan dijagnostički potencijal u razdvajanju obolelih od zdravih pacijentkinja, s' tim što je dijagnostički potencijal COPB2 gena specifičniji. Za Bcl-2 površina ispod krive (AUC) iznosi 0,666 i ovaj rezultat je statistički značajan (r=0,038). Za COPB2 površina ispod krive (AUC) iznosi 0,877 i ovaj rezultat je statistički značajan (r=0,000). Vrednosti relativne ekspresije COPB2 i Bcl-2 su snižene u početnim stadijumima karcinoma endometrijuma. Njihov poremećaj može imati značajnu ulogu u nastanku karcinoma endometrijuma.

SUMMARY: Genes responsible for the synthesis of proteins involved in cellular transport and metabolic processes play an important role in the development of endometrial carcinoma. Bcl-2 family proteins are key intracellular modulators of the apoptosis process. Previous studies have demonstrated the importance of COPB2 in the development and progression of adenocarcinoma. The aim of this study is to examine the impact of apoptosis disorders and COPB2 gene expression on the development of endometrial carcinoma. Endometrial tissue was taken from patients in the experimental group (40 patients) and the control group (20 patients). The relative expression of the COPB2 gene and Bcl-2 protein in endometrial cells was determined using the reverse transcription quantitative PCR method. The role of apoptosis regulation disorders via Bcl-2, as well as COPB2 gene expression in the development of endometrial carcinoma, was examined. It was found that there is a statistically significant difference (p<0.005) in COPB2 and Bcl-2 gene expression values between patients with and without endometrial cancer. For both genes, a significant diagnostic potential was shown in separating cancer patients from healthy patients, with the fact that the diagnostic potential of the COPB2 gene is more specific. For Bcl-2 area under the curve (AUC) is 0.666 and this result is statistically significant (p=0.038). For COPB2 the area under the curve (AUC) is 0.877 and this result is statistically significant (p=0.000). The relative expression values of COPB2 and Bcl-2 are decreased in the initial stages of endometrial carcinoma. Their disorder can play a significant role in the development of endometrial cancer.

karcinom endometrijuma, apoptoza, protein Bcl-2, COPB2, kvantitativni PCR.

endometrial carcinoma, apoptosis, protein Bcl-2, COPB2 quantitative PCR.

618.14-006(043.3)

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