Influence of strain differences between Dark Agouti and Albino Oxfors rats on the pathogenesis of different models of oral candidiasis

[M. V. Papić]

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Uvod: Oralna kandidijaza je infekcija sluzokože usne duplje koju izaziva oportunistički patogen, C. albicans. Najčešće nastaje u imunokompromitovanih, ali se može javiti i kod zdravih pojedinaca. Genske varijacije imaju uticaj na podložnost gljivičnim infekcijama i modulaciju imunskog odgovora. Dark Agouti (DA) i Albino Oxford (AO) pacovi su inbredni sojevi sa jasno determinisanim genskim profilima i razlikama u osetljivosti na brojne inflamatorne stimuluse i eksperimentalnu indukciju bolesti. Cilj: Ispitivanje sojnih razlika DA i AO pacova u indukciji, progresiji i tipu imunskog odgovora na oralnu kandidijazu izazvanu različitim eksperimentalnim modelima. Materijal i metode: Istraživanje je obuhvatilo 35 DA i 35 AO pacova, muškog pola, starosti 8 nedelja. Indukcija oralne kandidijaze je izvršena upotrebom tri eksperimentalna modela. Brisom je potvrđeno prisustvo C. albicans. Eksperiment je trajao 16 dana, nakon čega su životinje žrtvovane i prikupljeni uzorci krvi i tkiva jezika za histološku, imunohistohemijsku, qRT-PCR, ELISA i spektrofotometrijsku analizu. Rezultati: Veća kolonizacija C. albicans, veće oštećenje i veći inflamatorni infiltrat detektovan je u AO soju pacova. AO soj je pokazao veći broj CD45, CD3, CD68, ali i manji broj HIS 48 pozitivnih ćelija u odnosu na DA soj. Ekspresije IFN-γ, IL-1β, TNFα, TGF-β1 i IL-10 su bile manje u AO soju, dok je ekspresija IL-17 i IL-4 varirala između dva soja pacova zavisno od eksperimentalnog modela. Nivoi prooksidativnih parametara su bili veći kod AO pacova, dok su se nivoi antioksidativnih parametara razlikovali među sojevima zavisno od eksperimentalnog modela. Zaključak: DA i AO sojevi se razlikuju u odgovoru na infekciju C. albicans u okviru imunokompetentnih ili imunokompromitovanih stanja.

Introduction: Oral candidiasis is an infection of oral mucosa caused by the opportunistic pathogen, C. albicans. It most often occurs in immunocompromised, but it can also occur in healthy individuals. Genetic variations influence the susceptibility to fungal infections and immune response modulation. Dark Agouti (DA) and Albino Oxford (AO) rats are inbred strains with welldefined genetic profiles and differences in sensitivity to numerous inflammatory stimuli and experimental disease induction. Aim: To study the strain differences of DA and AO rats in the induction, progression and type of immune response to oral candidiasis induced by different experimental models. Material and methods: This research included 35 DA and 35 AO male rats, aged 8 weeks. Induction of oral candidiasis was performed using three experimental models. Swabs confirmed the presence of C. albicans. The experiment lasted 16 days, followed by sacrifice of animals and collection of blood and tongue samples for histological, immunohistochemical, qRT-PCR, ELISA and spectrophotometric analysis. Results: Greater C. albicans colonization, damage, and inflammatory infiltrate were detected in AO strain. A higher number of CD45, CD3, CD68 positive cells, and a lower number of HIS48 positive cells was present in AO compared to DA strain. The expressions of IFN-γ, IL-1β, TNFα, TGF-β1 and IL-10 were lower in AO strain, while the expression of IL-17 and IL-4 varied between two strains depending on the experimental model. Levels of pro-oxidative parameters were higher in AO rats, while the levels of antioxidative parameters differed between strains depending on the experimental model. Conclusion: DA and AO strains differ in response to C. albicans infection in both immunocompetent or immunocompromised states.

oralna kandidijaza, Dark Agouti, Albino Oxford, imunski odgovor, Candida albicans

oral candidiasis, Dark Agouti, Albino Oxford, immune response, Candida albicans

616.31-002.828(043.3)

Oralna medicina - Kandidijaza

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