The effects of modulation of creatine metabolism on heart function of rats exposed to high-intesity interval training

[V. Prokić]

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Uvod: Poslednjih godina, intervalni trening visokog intenziteta u kombinaciji sa različitim ergogenim sredstvima postao je veoma popularan modalitet treninga među sportistima. Cilj: Cilj ove studije je da se ispitaju efekti samostalne i udružene suplementacije kreatinom, betainom, gvanidinoacetatom kod treniranih pacova na funkciju srca, oksidacioni stres i na morfološku građu srca. Metode: U studiju je uključeno 72 pacova (Wistar albino soja, muškog pola, starosti 8 nedelja) razvrstani u 9 grupa: 1. KON- kontrola, 2. T- trening, 3. T+B – trening i betain, 4. T+K – trening i kreatin, 5. T+B+K – trening i betain i kreatin 6. T+G– trening i gvanidinoacetat, 7. T+G+K – trening i gvanidinoacetat i kreatin, 8. T+G+B – trening i gvanidinoacetat i betain, i 9. T+G+K+B – trening i gvanidinoacetat, kreatin i betain. Izolovano srce pacova je perfundovano metodom retrogradne perfuzije po Langendorff-u i praćeni su sledeći parametri: dp/dt max, dp/dt min, SLVP, DLVP i HR. U uzorcima koronarnog venskog efluenta i u krvi pacova određivani su parametri oksidacionog stresa. Izolovana srca, kvadriceps, jetra i bubrezi su ritinski pripremljeni za histološku analizu. Rezultati:. Suplementacija kreatinom, betainom i gvanidinoacetatom poboljšava funkciju miokarda, snižava vrednosti prooksidanasa a povećava vrednosti antioksidanasa. Najveći stepen hipertrofije kardiomicita, najveće povećanje depoa SERCA pumpe u srcu, kao i najveće smanjenje sadržaja kolagena u srcu bilo je u grupi životinja koje su trenirale i bile na suplementaciji gvanidinoacetatom. Zaključak: Suplementacija erogenim sredstvima kod treniranih pacova poboljšava kardiodinamske parametre i oksidacioni stres izolovanog srca pacova.

Introduction: In recent years, high-intensity interval training combined with various ergogenic means has become a very popular training modality among athletes. Objective: The aim of the study is to examine the effects of independent and combined supplementation with creatine, betaine, guanidinoacetate in trained rats on cardiac function, oxidative stress and morphological structure of the heart. Methods: The study included 72 rats (Wistar albino, male, 8 weeks old) divided into 9 groups: 1. KON- control, 2. T- training, 3. T+B- training and betaine, 4. T+K– training and creatine, 5. T+B+K– training and betaine and creatine 6. T+G– training and guanidinoacetate, 7. T+G+K– training and guanidinoacetate and creatine, 8. T+G+B– training and guanidinoacetate and betaine, and 9. T+G+K+B– training and guanidinoacetate, creatine and betaine. The isolated rat heart was perfused using the Langendorff retrograde perfusion method and the following parameters were monitored: dp/dt max, dp/dt min, SLVP, DLVP and HR. Oxidative stress parameters were determined in the samples of coronary venous effluent and in the blood of rats. Isolated hearts, quadriceps, livers and kidneys were routinely prepared for histological analysis. Results: Supplementation with creatine, betaine and guanidinoacetate improves myocardial function, lowers prooxidant values and increases antioxidant values. The highest degree of cardiomyocyte hypertrophy, the highest increase in the SERCA pump depot in the heart, as well as the highest decrease in collagen content in the heart was in the group of animals that trained and were supplemented with guanidinoacetate. Conclusion: Supplementation with erogenous agents in trained rats improves cardiodynamic parameters and oxidative stress in the isolated rat heart.

trčanje, kardiodinamika, kreatin, gvanidinoacetat, oksidacioni stres

running, cardiodynamics, creatine, guanidinoacetate, oxidative stress

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