The Effect of formulations based on Galium verum L. in the terapy of aphthous stomatitis in rats

[M. Lj. Vuletić]

108 listova

Uvod: Aftozni stomatitis je najčešće hronično oboljenje oralne mukoze. Postoje tri klinička oblika aftoznog stomatitisa u zavisnosti od karakteristika ulceracije. Terapija se najčećše ogleda u suzbijanju simptoma, i uglavnom se primenjuju antiseptički rastvori. Fitoterapija se već duže vreme primenjuje kao alternativna metoda u tretmanu aftoznog stomatitisa. Širok spektar dejstva Galium verum L. kao i uloga ove biljne vrste u zarastanju rana pruža osnov za potencijalnu primenu u terapiji aftoznog stomatitisa. Cilj: Cilj našeg istraživanja je bio ispitati efekat ekstrakta Galium verum L. u terapiji aftoznog stomatitisa kod pacova. Materijal i metode: U istraživanje je uključeno 80 pacova soja Wistar albino, kojima je indukovan aftozni stomatitis. Životinje su podeljene u četiri grupe. Razvoj i zarastanje lezija je praćeno svakodnevno. Žrtvovanje je sprovedeno 0., 3., 6. i 10. dana tretmana nakon čega su uzeti uzorci krvi i bukalnog tkiva kako bi se sprovele dalje analize. Efekti primenjenih formulacija su pored kliničke evaluacije, određeni histološkom analizom, praćenjem parametara inflamacije i oksidacionog stresa metodom kvantitativne reakcije lančane polimerizacije u realnom vremenu i spektrofotometrijski. Rezultati: Primena formulacija na bazi ekstrakta G. verum dovela je do značajnog smanjenja veličine lezije, patohistološkog skora i stepena inflamacije u aftoznim lezijama. Efekti primenjenih formulacija nisu imali uticaj redoks status u sistemskoj cirkulaciji dok je njihov uticaj u bukalnom tkivu bio značajan. Zaključak: Primena G. verum. u terapiji aftoznog stomatitisa ima značajnu ulogu u bržem zarastanju aftozne lezije i može služiti kao potencijalno sredstvo u terapiji oralnih ulceracija.

Introduction Aphthous stomatitis is the most common chronic oral ulcer disease. Three clinical types of aphthous stomatitis are described depending on characteristics of ulcer. The therapy is reflected in the suppression of symptoms, and antiseptics are usually used. Phytotherapy has been used for a long time as an alternative method in the treatment of aphthous stomatitis. The wide range of effects of the species Galium verum L. and it’s role in wound healing provides a basis for potential use in the therapy of aphthous stomatitis. Aim The aim of our research was to examine the effect of the extract of Galium verum L. in therapy of aphthous stomatitis in rats. Material and methods Research included 80 Wistar albino rats with aphthous stomatitis. The animals were divided into four groups. The evolvent and healing of the aphthous lesion were followed every day. Animals were sacrificed on day 0, 3, 6 and 10, and blood and buccal samples were collected for further analysis. In addition to clinical evaluation, the effects of the applied formulations were determined by histological analysis, by monitoring the parameters of inflammation and oxidative stress using PCR and spectrophotometric analysis. Results Application of formulations based on G. verum extract showed a significant reduction in ulcer size, pathohistological score and degree of inflammation in aphthous lesions. Applied formulations had no influence on systemic redox status, while their effects in the buccal tissue was significant. Conclusion Application of G. verum. in the therapy of aphthous stomatitis plays a significant role in reduction of healing time of the aphthous lesion and can serve as a potential tool in the therapy of oral ulcerations.

Aftozni stomatits, oralne ulceracije, Galium verum L..

Aphthous stomatitis; Oral ulcerations; Galium verum L.

616.31(043.3)

Tekst