Association of depression and anxiety with the expression of neurokinin receptors in women suffered from breast carcinoma

[M. B. Ilić]

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Karcinom dojke (KD) ostaje gorući globalni problem, kako po pitanju incidence tako i u pogledu mortaliteta. Poslednjih godina ulažu se napori u definisanju novih molekularnih i signalnih puteva uključenih u patogenezu KD, kako bi se dobila kompletnija i jasnija slika u pogledu predviđanja prognoze i efikasnosti primenjene terapije. Cilj ovog istraživanja je ispitivanje povezanosti anksioznosti i depresivnosti sa ekspresijom neurokininskih receptora (NKR) u karcinomu dojke, kao i njihova moguća veza sa kliničkim, patohistološkim i imunofenotipskim karakteristikama tumora. Istraživanje je podrazumevalo popunjavanje anketa za samoprocenu depresivnosti/anksioznosti obolelih žena, kao i kompletnu patohistološku analizu tkivnog materijala pacijentkinja. Na standardno obojenim preparatima definisani su svi značajni morfološki parametri KD. Imunohistohemijski, određivana je tkivna ekspresija relevantnih markera, primenom antitela (NKR, VEGF, CD105, Ki67 i caspase3). Definisane su dve grupe žena, sa i bez prisutnog depresivnog/anksioznog poremećaja, kao i postojeće razlike u pogledu kliničkih i histopatoloških karakteristika. Kod ispitivanih žena otkriven je značajan broj onih sa određenim stepenom depresivnosti/anksioznosti. Ekspresija NKR bila je u pozitivnoj korelaciji sa ispoljenim značajnim stepenom depresivnosti i sa ekspresijom značajnih prediktora ponašanja karcinoma poput markera proliferativnog indeksa Ki67, markera angiogeneze u tumoru VEGF i CD105, kao i markera apoptoze caspase-3. Nedvosmisleno je pokazano da je komorbiditet KD i depresije značajan u kliničkom pogledu, da postoje isprepletani molekularni mehanizmi čije bi podrobnije razumevanjem pomoglo na polju otkrivanja mesta za nove terapijske procedure koje bi ciljano delovale u sprečavanju rasta i invazije tumora.

Breast cancer (BC) remains a pressing global problem, both in terms of incidence and mortality. In recent years, efforts have been made to define new molecular and signaling pathways involved in the pathogenesis of BC, in order to obtain a more complete and clearer picture in terms of predicting the prognosis and the effectiveness of the applied therapy. The aim of this research is to examine the relationship between anxiety and depression with the expression of neurokinin receptors (NKR) in breast cancer, as well as their possible relationship with clinical, pathohistological, and immunophenotypic characteristics of the tumor. The research involved filling out questionnaires for self-assessment of depression/anxiety of the affected women, as well as a complete pathohistological analysis of the patient's tissue material. All significant morphological parameters of BC were defined on the standard stained preparations. Immunohistochemically, tissue expression of relevant markers was determined using antibodies (NKR, VEGF, CD105, Ki67, and caspase-3). Two groups of women were defined, with and without a present depressive/anxiety disorder, as well as existing differences in terms of clinical and histopathological characteristics. Among the examined women, a significant number of those with a certain degree of depression/anxiety were found. The expression of NKR was positively correlated with a significant degree of depression and with the expression of significant predictors of cancer behavior such as the proliferative index marker Ki67, tumor angiogenesis markers VEGF and CD105, as well as the apoptosis marker caspase-3. It has been unequivocally shown that the comorbidity of BC and depression is significant from a clinical point of view, that there are interwoven molecular mechanisms, the more detailed understanding of which would help in the field of discovering new therapeutic procedures that would act in a targeted manner in preventing tumor growth and invasion.

karcinom dojke; depresija; anksioznost; karcinogeneza; angiogeneza; metastaziranje; receptor za neurokinine NKR.

breast cancer; depression; anxiety; carcinogenesis; angiogenesis; metastasis; neurokinin receptor NKR.

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