Modulation of the cytokine profile of gingival fluid by the application of different approximal dental fillings

[V. B. Stefanović]

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Uvod: Dentalni karijes je jedno od najprevalentnijih oboljenja u ljudskoj populaciji, koje za posledicu ima i produkciju brojnih citokina. Materijal i metode: Utvrđivana je koncentracija citokina, metodom protočne citofluorimetrije, u GCF prema stepenu karijesa i vrsti primenjenog dentalnog materijala za privremenu ili trajnu restauraciju zuba kod aproksimalno lokalizovanih kaviteta. 90 ispitanika je podeljeno prema stepenu lezije karijesa i prema vrsti korišćenog DRM, a čiji su uzorci GCF uzimani su u 3 termina. Rezultati: Kod ispitanika grupe K III, pre terapije, u uzorcima GCF uočen je porast IL-2, IFN-γ i IL-4 a mesec dana nakon lečenja i porast IL-22, IL-1β i TNF-α. U K IV grupi pre restauracije detektovan je porast IL-9, a posle mesec dana porast IL-9 i IL-12. Kod K V grupe, pre započete terapije zabeležen je porast IL-12, IL-5, IL-10, IL-13, IL-22, IL-1β i TNF-α, a nakon mesec dana IL-5, IL-6, IL-10 i IL-13. Kod privremenih ispuna značajno je veći porast IL-2. Zaključak: Pre terapije, kod III stepena karijesa dominira profil Th1 odgovora, dok je Th2 profil detektovan kod V stepena karijesa. Različiti DRM indukovali su posebne profile citokina u GCF. Primena GJC kao i CPC je indukovala porast po 7 citokina, dok je primena CFC porast isključivo IL-9. Tretman sa BEA indukovao je značajan porast IL12, IFN- , IL-22 i IL-9. Ispuni sa AMA bili su povezani sa značajnim porastom IL-10 i TNF-, dok je TEC indukovao porast IL-10.

Introduction: Dental caries is one of the most prevalent diseases in the human population, which results in the production of numerous cytokines. Materials and Methods: The concentration of cytokines of were determined by flow cytofluorimetry in GCF according to the caries grade and the type of applied dental material for temporary or permanent tooth restoration in proximally localized cavities. 90 patients, whose GCF samples were taken in 3 appointments, were divided into groups according to the degree of caries lesion and the type of DRM used. Results: Among patients of the K III group, before therapy, an increase in IL-2, IFN-γ and IL-4 was observed in GCF samples, and a month after treatment, IL-22, IL-1β and TNF-α levels were increased as well. In the K IV group, an increase in IL-9 was detected before restoration, and an increase in IL-9 and IL-12 was observed after one month. In the K V group, before the start of therapy, an increase in IL-12, IL-5, IL-10, IL-13, IL-22, IL-1β and TNF-α was recorded, and after a month, IL-5, IL-6, IL-10 and IL-13 demonstrated higher levels. In the case of temporary fillings, there is a significantly higher increase in IL-2 levels. Conclusion: Before therapy, the Th1 response profile dominates in caries stage III, while the Th2 profile is detected in caries stage V. Different DRMs induced particular cytokine profiles in GCF. Application of GJC as well as CPC induced an increase in 7 cytokines each, while CFC application increased only IL-9. Treatment with BEA induced a significant increase in IL-12, IFN- , IL-22 and IL-9. Application of AMA materials increase in IL-10 and TNF-, while TEC induced an increase in IL-10.

karijes, dentalni materijali, gingivalna tečnost, protočna citofluorometrija, citokini

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