ǂThe ǂeffects of acute kidney injury induced by nephrotoxic agents on myocardial function in rats

[T. S. Nikolić]

89 listova

Cilj ovog istraživanja je da ispita uticaj akutnog oštećenja bubrega izazvanog nefrotoksičnim agesima: gentamicinom, diklofenom i folnom kiselinom na funkciju miokarda i inflamacijsko-oksidacioni status pacova. Istraživanje je obuhvatilo 100 pacova Wistar albino soja muškog pola starosti 6 nedelja, prosečne telesne mase oko 200 grama. Pacovi su podeljeni u tri eksperimentalne grupe od kojih je svaka tretirana različitim nefrotoksičnim agensom: diklofenom, gentamicinom i folnom kiselinom i kontrolnu koja je tretirana fiziološkim rastvorom. Nakon eksperimentalnog tretmana i žrtvovanja sprovedena su ispitivanja na izolovanom srcu metodom po Langendorff-u praćenjem parametara srčane funkcije: dp/dt max, dp/dt min, SLVP, DLVP, HR i CF, dok su spektrofotometrijskim metodama u efluentu i plazmi određeni parametri oksidacionog stresa (TBARS, NO2 - , O2 - , H2O2) i u lizatu eritrocita parametri antioksidacione zaštite (CAT, SOD, GSH). ELISA metodom u plazmi određeni su biomarkeri inflamacije (TNF-α, IL-1, IL-6). Rezultati ove studije pokazuju da akutno oštećenje bubrega utiče na kardijalnu hemodinamiku, sistemska i kardijalna oksidacijska svojstva kao i na sistemsku inflamaciju i pomaže u rasvetljavanju efekata akutnog oštećenja bubrega na funkciju i redoks potencijal kardiovaskularnog sistema.

The aim of this study was to examine the effect of acute kidney injury caused by nephrotoxic ages: gentamicin, diclofen and folic acid on myocardial function and the inflammatory-oxidative status of rats. The study included 100 ѕix week old male Wistar albino rats, with an average body weight about 200 grams. Rats were divided into three experimental groups, each of them were treated with a different nephrotoxic agent: diclofenac, gentamicin and folic acid, and a control group treated with saline. After experimental treatment and sacrifice, studies on the isolated heart were performed by the Langendorff method by monitoring the parameters of cardiac function: dp / dt max, dp / dt min, SLVP, DLVP, HR and CF. The parametres of oxidative stress (TBARS, NO2-, O2-, H2O2) in effluent and plasma were determined by spectrophotometric methods as well as parameters of antioxidant protection (CAT, SOD, GSH) in erythrocyte lysate. Biomarkers of inflammation in plasma (TNF-α, IL-1, IL-6) were determined by ELISA method. The results of this study show that acute renal impairment affects cardiac hemodynamics, systemic and cardiac oxidative properties as well as systemic inflammation and helps to elucidate the effects of acute renal impairment on the function and redox potential of cardiovascular system.

akutno oštećenje bubrega, izolovano srce pacova, kardiovaskularni sistem, oksidacioni stres, inflamacija

acute kidney injury, isolated rat heart, cardiovascular system, oxidative stress, inflamation

599.323.4:616.61(043.3)

Tekst